联系我们
分享至
Intended Use
Porcine Circovirus Type-Ⅱ Antibody ELISA Detection Kit is for the determination of specific PCV antibody in pig’s serum in vitro.
The test is used to assess the vaccinated from immunization with porcine circovirus type--Ⅱ vaccine of pig farms.
Principle
The test is prepackaged with PCV antigen in micropores of enzyme-labeled lath using indirect ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains anti-PCV specific antibody, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, the enzyme-labeled conjugate is added to bind with specific antigen-antibody complex on the coated plate. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader.
立即咨询
我们将在一个工作日内与您联系，请留意您的电子邮件。