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Intended Use
Infectious Bursal Disease Virus Antibody ELISA Detection kit is used to determine the Infectious Bursal Disease Virus antibodies in chicken serum in vitro.
Principle
The test is prepackaged with Bovine infectious rhinotracheitis antigen in micropores of enzyme-labeled lath using indirect ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains antibodies against infectious bovine rhinotracheitis virus, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, enzyme-labeled antibodies are added to combine with the antigen-antibody complex in the well to form an antigen-antibody-enzyme-labeled antibody complex. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader.
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