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Intended Use
The Bovine Tuberculosis Antibody ELISA Detection Kit is for the determination of bovine tuberculosis antibody in bovine’s serum of Mycobacterium bovis in vitro.
The test is used to assess the infection of bovine tuberculosis. MPB70-MPB83 chimera recombinant was applied in this test.
Principle
The test is prepackaged with bovine tuberculosis antigen in micropores of enzyme-labeled lath using indirect ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains anti-BT specific antibody, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, the enzyme-labeled conjugate is added to bind with specific antigen-antibody complex on the coated plate. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was inversely proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader.
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