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Intended Use
The Porcine Pseudorabies Virus gE Blocking Antibody ELISA Detection Kit is for the determination of gE antibody in pig’s serum of porcine pseudorabies virus in vitro.
The test is used to distinguish infected wild virus and vaccinated from immunization with gE gene deletion vaccine.
Principle
The test is prepackaged with PRV-gE antigen in micropores of enzyme-labeled lath using blocking ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains anti-PRV-gE specific antibody, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, the enzyme-labeled PRV-gE antibody is added, and the antibody in the sample blocks the binding of the enzyme-labeled antibody to the antigen on the coated plate. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was inversely proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader
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