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Intended Use
The Clostridium perfringens Ag Test is a lateral flow immunochromatographic assay designed for detection of alpha-toxin generated by Clostridium perfringens (CPE Ag) in equine feces. It offers two options for result reading: visual judgment or semi-quantitative analysis with the FIC-Q100N Dry Immunoassay Analyzer (required only for Method 2).
Principle
The test card features a testing window for observing assay progress and result reading. This window contains an initially invisible T (test) zone and a C (control) zone. In the assay, highly selective antibodies to Clostridium perfringens antigen serve as the capture (T) and detector (gold-labeled). When the treated sample is added to the sample well on the test card, the liquid flows laterally across the test strip. If Clostridium perfringens antigen is present in the specimen, a visible T line appears, which can also be recognized by the FIC-Q100N Dry Immunoassay Analyzer. The C line must always appear after sample application, indicating proper test performance. Thus, the test card accurately indicates the presence of Clostridium perfringens antigen in the specimen.
Instruments suitable
Suitable for VETSEE G2 DRY IMMUNOASSAY ANALYZER
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